期刊论文

Effect of Serum and Plasma Containing Wuhu Decoction In vitro on T cell Proliferation and Activation through Intervening RSV-Induced Dendritic Cells Autophagy

中文

中药药理与临床

1001-859X

2020

36

05

65-73

10.13412/j.cnki.zyyl.2020.05.001

国家自然科学基金资助项目（编号:81774368）； 湖南省自然科学基金科卫基金项目（编号:2018JJ6114）； 湖南省研究生科研创新资助项目（编号:CX20190580）； 湖南中医药大学中医学国内一流学科建设学科项目（编号:4901-020002002）；

本校为第一机构

目的:观察五虎汤含药血浆与含药血清体外干预呼吸道合胞病毒(Respiratory syncytial virus,RSV)诱导的树突细胞(dendritic cells,DCs)自噬对T细胞增殖活化的影响,为揭示五虎汤治疗病毒性哮喘的机制及体外实验选择合适的研究方法提供参考依据。方法: SD大鼠予2.2 g/kg五虎汤灌胃7 d后分别制得含药血清与含药血浆,从BaLB/C小鼠骨髓中分离诱导DCs细胞,从OT2小鼠脾脏中分离CD4~+T细胞,在Transwell系统里以1 ∶ 10的比例共培养,随机分为正常对照血浆组、模型血浆对照组、4.4 g/kg五虎汤5%、10%、20%含药血浆组、正常对照血清组、模型血清对照组、4.4 g/kg五虎汤5%、10%、20%含药血清组及雷帕霉素10 μ...

Objective: To observe the effect of serum and plasma containing Wuhu decoction in vitro on T cell proliferation and activation through intervening RSV-induced Dendritic cells (DCs) autophagy. To reveal the mechanism of Wuhu decoction in treating viral asthma and provide the reference for appropriate research methods in vitro. Methods: Drug-containing serum and Drug-containing plasma were prepared after SD rats were given (ig) 2.2 g/kg Wuhu decoction for 7 days. DCs were isolated from bone marrow of BaLB/C mice. CD4~+T cells were isolated from t...