期刊论文

Shenfu Injection Improves Water and Sodium Metabolism in Rats with Heart Failure by Vasopressin Escape

中文

中药药理与临床

1001-859X

2020

36

06

57-62

10.13412/j.cnki.zyyl.2020.06.005

81503627:国家自然科学基金 81774208:国家自然科学基金 81973753:国家自然科学基金 18K070:湖南省教育厅科学研究项目 B2015-07:湖南省保健专项 2015092:湖南省中医药局科研项目

本校为第一机构

目的:通过参附注射液作用于异丙肾上腺素诱导的心衰大鼠模型以证实其是否可通过血管加压素(AVP)逃避改善心衰后的水液代谢。方法:除空白对照组外,其余大鼠用异丙肾上腺素制作心衰模型,监测大鼠尿量、体重,随后将成模大鼠随机分为:模型对照组、参附10 ml/kg组、螺内酯20 mg/kg组、参附20 ml/kg+螺内酯20 mg/kg组、参附10 ml/kg+螺内酯20 mg/kg组、参附5 ml/kg+螺内酯20 mg/kg组。大鼠处死前,从主动脉采血,检测大鼠血管加压素(AVP)和N-末端脑钠肽前体(NT-proBNP)水平;通过荧光定量PCR和Western Blot对大鼠肾髓质水通路蛋白-2(AQP-2)、血管加压素受体-2(V_(2)R)的mRNA和蛋白表达进行检测。结果:造模...

Objective: To study the influence of Shenfu Injection on isoproterenol induced heart failure to confirm its effect of escaping from vasopressin (AVP) to improve the water metabolism after heart failure in rats. Methods: Except for the control group,the heart failure model was induced by isoproterenol injection,then the urine volume and the body weight were monitored. The model rats were randomly divided into the model group,10 ml/kg Shenfu group,20 mg/kg spironolactone group and the combination groups (20 ml/kg,10 ml/kg and 5 ml/kg Shenfu + 20 mg/kg spironolactone). Before the rats were killed...