期刊论文

Effects of Yinxing Yangnao Tablet(银星养脑片)in Treatment of Vascular Dementia in Rats Based on UHPLC-QE-MS by Serum Metabolomics

中文

中药药理与临床

1001-859X

2021

37

06

111-119

湖南省教育厅优秀青年项目（编号：20B441）； 湖南省科技厅优秀青年项目（编号：2019JJ50445）； 湖南省自然科学基金项目（编号：2021JJ80070）； 湖南省卫健委项目（编号：202213054508）； 校级重点项目（编号：2019XJJJ076）；

本校为第一机构

目的:基于超高效液相色谱-质谱联用（UHPLC-QE-MS）技术探讨银星养脑片对血管痴呆性大鼠血清中内源性标志物的影响,并初步探讨其发挥药效的作用通路。方法:采用双侧颈总动脉结扎法诱导血管性痴呆模型,将SD大鼠随机分为正常对照组、模型对照组、吡拉西坦片0.4 g/kg组、银星养脑片0.27 g/kg组,每组10只。经双侧颈总动脉结扎后14 d开始给药,连续给药14 d,给药结束后立即进行水迷宫测试并将动物脑组织进行HE染色,应用UHPLC-QE-MS技术进行血清样品的分离和数据采集,采用主成分分析法（PCA）和正交偏最小二乘判别分析法（OPLS-DA）筛选出相关差异代谢物,并对已经鉴定的代谢标志物使用KEGG数据库进行可...

Objective:To investigate the effects and the underlying mechanism of Yinxing Yangnao Tablet on the potential endogenous markers of vascular dementia rats by UHPLC-QE-MS,and explore its effective pathways.Methods:The vascular dementia model was induced by permanent ligation of bilateral common carotid arteries.SD rats were randomly divided into a control group,a model group,a piracetam group(0.4 g/kg),and a Yinxing Yangnao Tablet group(0.27 g/kg),with 10 rats in each group.Fourteen days after ligation of bilateral common carotid arteries,drugs were orally administered for 14 days.After the last...