Traditional Chinese Medicine (TCM) holds significant potential as a natural source of medication, providing effective alternatives for preventing and treating thrombotic diseases. Although research on the anticoagulant activity of Agrimonia Pilosa Ledeb (Rosaceae, A. pilosa) has been paid attention to, the monomer component and its mechanism of action have not been studied deeply. Therefore, this study aimed to screen and identify the antithrombotic active components of A. pilosa by affinity ultrafiltration (AUF) combined with ultra-high performance liquid chromatography-quadrupole time-of-fli...