会议论文

中文

2012

515-518

第二十九次全国中医儿科学术大会

第二十九次全国中医儿科学术大会论文集

2012-09-01

张家界

中华中医药学会

本校为第一机构

目的:探讨咳喘宁口服液对RSV诱发哮喘CD4~+T、CD8~+T细胞内PI3K表达的影响,进一步阐明咳喘宁口服液防治病毒诱发哮喘的机理。方法:将40只幼年SD大鼠随机分为4组,分别为磷酸盐缓冲液(PBS)对照组、模型组、地塞米松+沙丁胺醇组和咳喘宁组。建立卵白蛋白哮喘大鼠模型并以呼吸道合胞病毒激发哮喘。采用磁珠结合法分离CD4~+T、CD8~+T细胞,免疫荧光法,使用流式细胞仪检测T细胞内PI3K

　　Purpose:To research the effect of Kechuanning Oral liquid on the expression of P13K in the cells ofCD4+T、CD8+T about RSV-induced asthma,and to further elucidate the mechanism of Kechuanning Oral liquidpreventing and treating the asthma induced by virus.Methods:Totally infancy 40 rates were randomly divided intofour groups,these are phosphate-buffered saline(PBS)control group,model group,dexamethasone with salbutamolgroup,and Kechuanning Oral liquid group.We builded rat model of asthma induced by ovalbumin and stimulatedasthma with respirat...