期刊论文

中文

癌变·畸变·突变

1004-616X

2013

25

3

183-189

10.3969/j.issn.1004-616x.2013.03.005

81273988:国家自然科学基金 81072904:国家自然科学基金 2011M501277:中国博士后科学基金 2011:高等学校学科创新引智计划计划) :高等学校学科创新引智计划计划) :湖南省中医五官科学重点学科建设项目

本校为第一机构

目的: 研究下行型鼻咽癌不同中医临床证型患者原发病灶组织细胞核差异表达蛋白质,为中医证型分类的科学性提供依据。方法:采用双向凝胶电泳及基质辅助激光解吸电离-飞行时间质谱法(MALDI-TOF-MS)对下行型鼻咽癌火毒困结型、气阴两虚型和气血凝结型患者的鼻咽癌组织细胞核蛋白进行分离和表达谱研究,利用生物信息学方法鉴定差异蛋白质。结果:与下行型鼻咽癌气阴两虚型细胞核蛋白点相比,气血凝结型有28个蛋白点表达上调、12个蛋白点表达下调,火毒困结型有9个点表达上调,19个蛋白点表达下调;与下行型鼻咽癌气血凝结型细胞核蛋白点相比,火毒困结型有25个蛋白点表达上调、18个蛋白点表达下调。MALDI-T...

OBJECTIVE： To study the different nuclear protein expression in primary focus of downward progressive type of nasopharyngeal carcinoma in patients with different clinical patterns according to TCM classification. METHODS：Nuclear proteins profiles in fire-toxin stagnation type,Qi-yin deficiency type and Qi-blood coagulation type were measured by two-dimensional gel electrophoresis and matrix assisted laser. RESULTS：Compared with the expression of nuclear protein spots from patients with Qi-yin deficiency type,there were 28 up-regulated protei...