期刊论文

Liu, Q.-L.

中文

中国组织工程研究

2095-4344

2005

9

19

146-147

10.3321/j.issn:1673-8225.2005.19.079

湖南省教育厅资助课题（02C291）~~；

本校为第一机构

目的:观察还少丹对老年小鼠衰老指标活性氧损害程度与DNA端粒长度的影响.方法:实验于2005-01/04在湖南中医学院分子生物学实验室完成,选择24只健康昆明种老年小鼠,随机分为对照组和药物组,各12只.药物组用还少丹水煎液(由何首乌、熟地、肉苁蓉等中药组成)灌胃10 μL(含生药10 g)/(g·d),对照组用等量生理盐水灌胃,每周灌胃5 d,共给药70 d.小鼠断头取血,离心分离出红细胞,加蒸馏水制成溶血液.溶血液中超氧化物歧化酶含量采用光化学扩增法检测,溶血液中过氧化氢酶含量以过氧化氢分解法检测,同时采血测定溶血液血红蛋白的含量.小鼠肝组织DNA的平均端粒长度应用Southern印迹杂交检测.结果:实验过程中...

Aim: To observe the effects of huanshao dan on the aging indexes of damaged severity of active oxygen and length of DNA telomere in mice. Methods: The experiment was carried out in the Laboratory of Molecular Biology, Hunan College of Traditional Chinese Medicine from January to April 2004. Twenty-four healthy Kunming aging mice were randomly divided into control group(n = 12) and drug group(n = 12). Mice in the drug group were treated with gastric perfusion of huanshao dan decoction containing 10 g crude drugs(10 μL/g per day), which consiste...