【Objective】To study the influence of transdermal enhancers on percutaneous absorption of baicalin in eczema spray and to get optimum concentration.【Methods】The test for baicalin permeation through large white rat ventral skin in vivo was performed in a special improved Franz diffusion cell,the amounts of baicalin of this permeation were determined with a first derivative spectrum method.Influence of different concentration of azone and propylene glycol on the permeation of baicalin through rat skin in vitro was observed.【Results】Finally,t...