期刊论文

Huang, Yaliang;Liu, L

英文

Talanta

0039-9140

2025

281

126796

10.1016/j.talanta.2024.126796

CRediT authorship contribution statement Suling Yang: Writing – original draft, Investigation, Conceptualization. Yaliang Huang: Writing – review & editing, Methodology, Investigation. Tingting Yang: Investigation. Jingze Li: Investigation. Jiaxin Tian: Investigation. Lin Liu: Writing – review & editing, Project administration, acquisition, Conceptualization.

本校为其他机构

Poly(ADP-ribose)polymerase-1 (PARP1) could be activated by binding to nucleic acids with specific sequences, thus catalyzing the poly-ADP-ribosylation (PARylation) of target proteins including PARP1 itself. Most of the previously reported electrochemical methods for the determination of PARP1 were relied on the electrostatic interactions, which required the pre-immobilization of DNA on an electrode for the capture of PARP1. Herein, we reported an "immobilization-free" electrochemical strategy for the assays of PARP1 on the basic of avidin-biotin interaction. Once PARP1 was activated by binding...