期刊论文

Effects of C1GALT1 and Cosmc mRNA Expression of Liuwei Dihuang Decoction on Ig A Nephropathy Rat Peripheral Blood

中文

中国中西医结合肾病杂志

1009-587X

2016

17

3

211-214

湖南省科技厅基金资助项目（No.2013SK3099）；

本校为第一机构

目的：观察六味地黄汤对Ig A肾病大鼠外周血C1GALT1及Cosmc mRNA表达,探讨其对Ig A糖基化的影响。方法：SD大鼠40只随机分为正常对照组,模型组,雷公藤多苷片组,六味地黄汤组。除正常对照组外均采用运用牛血清白蛋白＋脂多糖＋四氯化碳方法建立实验性Ig A肾病模型。造模成功后连续灌胃4周。将各组大鼠外周血分离的PBMC悬液,采用实时荧光定量PCR检测C1GALT1及Cosmc mRNA的表达,ELISA法测定外周血中Ig A含量,凝集素亲和ELISA法检测Ig A1异常糖基化程度,并进行定量分析。结果：各组C1GALT1mRNA表达量并差异无统计学意义（P〉0.05）;在各组Cosmc mRNA表达中,六味地黄汤组及雷公藤多苷片组表达均较模型...

Objective:To observe the effects of Liuwei Dihuang Decoction on IgA nephropathy rat peripheral blood C1GALT1 and Cosmc mRNA expression, and to investigate its effect on IgA glycosylation. Methods:Forty SD rats were equally ran-domized into 4 groups: the normal group, model group, tripterygium glycosides group, Liuwei Dihuang decoction group. IgAN rat model was established with bovine serum albumin (BSA), lipopolysaccharide (LPS), carbon tetrachloride (CCl4). After 4 weeks of continuous perfusion. Each group of rat peripheral blood separation of PBMC suspension, using real-time fluorescent quan...