期刊论文

Effect of Xinkang Granule on Anti-Myocardial Fibrosis by Regulating miRNA-21/PTEN in Rats with Chronic Heart Failure

中文

湖南中医药大学学报

1674-070X

2018

38

3

270-274

10.3969/j.issn.1674-070X.2018.03.010

湖南省自然科学基金项目（2016JJ4068）； 国家重点实验室中医诊断学开放基金（2015ZYZD11）； 湖南省中医药管理局项目（201610）；

本校为第一机构

目的探讨心康冲剂抗心衰大鼠心肌纤维化的机制。方法90只SD大鼠分为正常组10只,模型组、对照组及心康低、中、高剂量组各16只。心康低、中、高剂量组分别给予0.5、1、2 g/（kg·d）的心康冲剂溶液。对照组给予0.06 g/（kg·d）的芪苈强心胶囊。正常组、模型组按1 m L/（kg·d）灌服生理盐水。各组均每日1次,连续灌服8周。计算左心室质量指数（LVMI）;心脏切片行HE、Masson染色并计算胶原容积分数（CVF）;采用Real-time PCR法检测miRNA-21、人第10号染色体缺失的磷酸酶及张力蛋白同源基因（PTEN）m RNA表达水平;用Pearson相关及Logistic多元线性回归分析miRNA-21、PTEN m RNA、CVF与LVMI之间的相...

Objective To explore the mechanism of Xinkang Granule on anti-myocardial fibrosis in rats with heart failure Methods Ninety SD rats were randomly divided into normal group (n=10), model group (n=16), control group (n=16), XKG low-dose group (n=l6), XKG middle-dose group (n=l6), XKG high-dose group (n=16). Xinkang groups were given with 05 1,2 g/(kg·d) Xinkang granule solution. The control group was given with 0.06 g/(kg·d) Qiliqiangxin capsule (QLQXC). Rats in the normal group and model control group were administrated with saline at 1 mL/(kg...