期刊论文

Experimental Study on Inhibitory Effect on the Proliferation of Hep G2 Cells with Three Different Herbs

中文

中医药导报

1672-951X

2014

20

12

29-32

10.13862/j.cnki.cn43-1446/r.2014.12.011

本校为第一机构

目的:探讨3种中药制剂肝复乐、黄芪注射液、β-榄香烯对Hep G2细胞增殖的抑制作用。方法:长满单层的对数生长期HepG2细胞,分别加入用DMEM完全培养基进行连续二倍稀释的中药复方肝复乐,以及黄芪注射液、β-榄香烯注射液,并设环磷酰胺药物对照与细胞空白对照,于37℃5%CO2分别培养48 h,采用MTT法测药物对Hep G2细胞的增殖、生长的抑制作用。结果:肝复乐、黄芪注射液、β-榄香烯对Hep G2细胞有明显的抑制作用,与空白对照组及环磷酰胺比较,差异均有统计学意义（P

Objective： To study the inhibitory effect on the proliferation of Hep G2 cells with GanFuLe, Astragalus injection and β-Elemene. Methods： Covered with a monolayer of Hep G2 cells in logarithmic growth phase were added to DMEM complete medium with continuous double dilution GanFuLe, Astragalus injection,β-Elemene injection, and set comparison among cyclophosphamide drug control and blank control, cells at 37℃5% CO2 were cultured 48 h, MTT assay was used to measure the drug Hep G2 ceils proliferation, inhibition of growth. Results： Compared...