期刊论文

中文

中国骨质疏松杂志

1006-7108

2016

22

4

387-392,395

10.3969/j.issn.1006-7108.2016.04.001

国家自然科学基金

本校为第一机构

目的观察补骨脂素对去势雌鼠E_2、ER_β、TNF-α、IL-17的影响,探讨补骨脂素防治绝经后骨质疏松的机理。方法选取3月龄雌性SD大鼠72只,随机分为假手术组、模型组、戊酸雌二醇对照组、壮骨止痛胶囊对照组、补骨脂素高剂量组、补骨脂素低剂量组,每组12只。除假手术组外,其它大鼠造成双侧去卵巢骨质疏松症病理模型,术后5d拆线后开始给药,连续13周。最后一次给药后次日取血标本采用ELISA检测血清中雌激素(E_2) 、肿瘤坏死因子-α(TNF-α) 、白细胞介素-17(IL-17)的含量。提取左股骨组织mRNA样本进行RT-PCR反应检测骨组织TNF-α、ER_β、IL-17基因的表达。取右股骨脱钙切片免疫组化检测ER_β表达。结...

Objective To investigate the anti-osteoporosis mechanism of psoralen by observing its effect on E_2,ER_β,TNF-α, and IL-17 in ovariectomized rats. Methods Seventy-two 3-month-old SD female rats were randomly allocated into sham operation group,model group,estradiol group,Zhuanggu Zhitong (ZGZT) capsule group,the high psoralen dosage group,and the low psoralen dosage group,with 12 rats in each group. Except the rats in the sham operation group,the rats in the other groups were ovariectomized bilaterally. The medicines were given to rats via int...