Simple, rapid, and environmentally friendly method for the separation of isoflavones using ultra-high performance supercritical fluid chromatography

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成果类型：

期刊论文

作者：

Wu, Wenjie;Zhang, Yuan;Wu, Hanqiu;Zhou, Weie;Cheng, Yan;...

通讯作者：

Zhang, Feng

作者机构：

[Cheng, Yan; Li, Hongna; Wu, Hanqiu; Wu, Wenjie; Zhang, Yuan; Zhou, Weie; Zhang, Chuanbin; Zhang, Feng] Chinese Acad Inspect & Quarantine, Inst Food Safety, Beijing, Peoples R China.

[Wu, Wenjie; Li, Lulu; Huang, Ying] Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China.

[Zhang, Yuan] Chinese Acad Med Sci, Canc Hosp, Natl Canc Ctr, Dept Pharm, Beijing, Peoples R China.

[Zhang, Yuan] Peking Union Med Coll, Beijing, Peoples R China.

通讯机构：

[Zhang, Feng] C

Chinese Acad Inspect & Quarantine, Inst Food Safety, Beijing, Peoples R China.

语种：

英文

关键词：

isoflavones;separation factors;ultra high performance supercritical fluid chromatography

期刊：

Journal of Separation Science

ISSN：

1615-9306

年：

2017

卷：

期：

页码：

2827-2837

DOI：

10.1002/jssc.201601454

基金类别：

National Science and Technology Major Project of "Study on the Quality Detection Technology for Import and Export Medicine and Food Dual Purposes Products" [YS2017YFZG020006]

机构署名：

本校为其他机构

院系归属：

药学院

摘要：

Isoflavones are natural substances that exhibit hormone-like pharmacological activities. The separation of isoflavones remains an analytical challenge because of their similar structures. We show that ultra-high performance supercritical fluid chromatography can be an appropriate tool to achieve the fast separation of 12 common dietary isoflavones. Among the five tested columns the Torus DEA column was found to be the most effective column for the separation of these isoflavones. The impact of individual parameters on the retention time and separation factor was evaluated. These parameters wer...

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Simple, rapid, and environmentally friendly method for the separation of isoflavones using ultra-high performance supercritical fluid chromatography

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