Tissue-specific metabolite profiling of benzylisoquinoline alkaloids in the root of Macleaya cordata by combining laser microdissection with ultra-high-performance liquid chromatography/tandem mass spectrometry
Project in the National Science & Technology Pillar Program [2012BAI29B04]; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31200615, 31600238]
机构署名：
本校为其他机构
院系归属：
第二中医临床医学院
摘要：
Rationale: Tissue-specific metabolite profiling helps to find trace alkaloids masked during organ analysis, which contributes to understanding the alkaloid biosynthetic pathways in vivo and evaluating the quality of medical plants by morphology. As Macleaya cordata contains diverse types of benzylisoquinoline alkaloids (BIAs), the alkaloid metabolite profiling was carried out on various tissues of the root. Methods: Laser microdissection with fluorescence detection was used to recognize and dissect different tissues from the root of M. cordata....
