期刊论文

中文

基础医学与临床

1001-6325

2017

37

12

1663-1667

10.16352/j.issn.1001-6325.2017.12.001

81373578:国家自然科学基金 81573965:国家自然科学基金 81403379:国家自然科学基金 81403387:国家自然科学基金

本校为第一机构

目的探讨S100B、AQP4和CX43表达紊乱对糖尿病并发抑郁症大鼠海马血脑屏障功能的影响。方法将大鼠分为糖尿病组[高脂乳灌胃2周+尾静脉注射链脲佐菌素(STZ,38 mg/kg)]、抑郁症组(慢性不可预知性应激28 d)、糖尿病并发抑郁症组(联合以上两种方法建立)、对照组。造模结束后,旷场实验和Morris水迷宫实验观察大鼠行为学,ELISA检测血清S100B含量,免疫组化法检测海马血脑屏障AQP4和CX43的表达。结果与对照组比较,糖尿病组和抑郁症组大鼠自主活动次数和空间探索时间显著减少,逃避潜伏期延长(P

Objective To investigate the effects of S 100B, AQP4, and CX43 on the function of the blood brain barrier in the hippocampus of diabetic rats with depression .Methods Rats were divided into the diabetic group [ two weeks of high-fat diet and injection of Streptozotocin (STZ, 38 mg/kg)], depression group(chronic unpredictable stress for 28 days) , the diabetes mellitus with depression group ( combined with the above two methods ) , and the control group .The behavior of rats was evaluated with open-field test and Morris test .The expressions of AQP 4 and CX43 in rat hippocampus blood-brain barr...