版权说明 帮助中心
首页 > 成果 > 详情

下丘脑-垂体-肾上腺轴紊乱对焦虑性抑郁模型大鼠海马结构的影响

CSCD
认领
导出
Link by 中国知网学术期刊 Link by 维普学术期刊 Link by 万方学术期刊
反馈
分享
QQ微信 微博
成果类型:
期刊论文
论文标题(英文):
Effect of hypothalamus pituitary adrenal axis disorder on hippocampal formation in rats with anxious depression
作者:
凌佳;吴梦瑶;杨琴;赵洪庆;杜青;韩远山;王宇红
作者机构:
[凌佳; 吴梦瑶; 赵洪庆; 王宇红] 湖南中医药大学, 湖南省中药粉体与创新药物省部共建国家重点实验室培育基地, 湖南, 长沙, 410208
[杨琴; 韩远山; 王宇红] 湖南中医药大学第一附属医院, 湖南, 长沙, 410007
[杜青] 湖南省中医药研究院中药研究所, 湖南, 长沙, 410013
湖南中医药大学, 湖南省中药粉体与创新药物省部共建国家重点实验室培育基地, 湖南, 长沙, 410208
湖南中医药大学第一附属医院, 湖南, 长沙, 410007
语种:
中文
关键词:
焦虑性抑郁;焦虑性抑郁模型;HPA轴紊乱;病理变化;糖皮质激素受体
关键词(英文):
anxious depression;anxious depression model;HPA axis disorder;pathological change;glucoeorticoid receptor
期刊:
基础医学与临床
期刊(英文):
Basic & Clinical Medicine
ISSN:
1001-6325
年:
2018
卷:
38
期:
9
页码:
1231-1238
基金类别:
国家自然科学基金(81573965,81403387); 湖南省科技厅重点项目(2015DK3003); 校研究生创新课题(2017CX30);
机构署名:
本校为第一机构
院系归属:
药学院
湖南省中医药研究院
摘要:
目的研究下丘脑-垂体-肾上腺(HPA)轴紊乱对焦虑性抑郁模型大鼠海马结构的影响,探讨焦虑性抑郁的潜在发病机制。方法将大鼠随机分为空白组、溶媒组、焦虑组、抑郁组和焦虑性抑郁组,每组12只。采用慢性束缚应激联合皮质酮注射方法建立焦虑性抑郁大鼠模型,连续21 d;造模后采用高架十字迷宫(EPM)、旷场实验(OFT)、强迫游泳实验(FST)评价大鼠焦虑和抑郁样行为;HE染色检测大鼠HPA轴各组织及海马病理变化;ELISA检测大鼠血浆中促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质酮(CORT)含量;Western blot检测大鼠海马糖皮质激素受体(GR)蛋白表达。结果焦虑性抑郁组大鼠进入开臂的时间、次数及自主活动次数均与焦虑组相当,不动时间显著增加,与对照组及抑郁组比较有显著差异(P<0.01或P<0.05);HPA轴各组织均出现不同程度损伤,海马神经元肿胀,呈空泡状;同时,血浆中CRH、ACTH和CORT含量显著增加(P<0.01或P<0.05),海马GR表达显著下降。结论焦虑性抑郁模型组大鼠具有显著的焦虑及抑郁样行为,其发病机制可能与机体HPA轴紊乱及其引发的脑内海马损伤密切相关。
摘要(英文):
Objective To study the effect of hypothalamus pituitary adrenal(HPA) axis disorder on the hippocampal formation in rats with anxious depression and to explore its potential pathogenesis. Methods Rats were randomly divided into control group, vehicle group, anxiety group, depression group and anxious depression group with 12 rats in each group. Anxious depression rats were intervened by chronic restraint stress combined with corticosterone injection for continuous 21 d. Then, elevated plus maze (EPM), open field test (OFT), forced swimming test(FST)were used to evaluate the anxious and depression like behavior in rats, HE staining to check pathological changes of the HPA axis tissue and hippocampus. The plasma levels of CRH, ACTH and CORT of rats were detected by ELISA, and the expression of GR in hippocampus was detected by Western blot. Results Compared with control group and depression group, there were significant differences (P<0.01 or P<0.05) in OE% and OT% at EPM and independent activity frequency during OFT, which was similar to anxious depression group. Compared with control group and anxious group, the immobility time in FST was increased significantly (P<0.01). HE staining showed that all tissues of HPA axis were damaged, and the hippocampal neurons were swollen and vacuolated. ConclusionsAnsious depression rats have significant anxious and depressive behaviors, its pathogenesis may be closely related to the HPA axis disorder and hippocampus region damage.

反馈

验证码:
看不清楚,换一个
确定
取消

成果认领

标题:
用户 作者 通讯作者
请选择
请选择
确定
取消

提示

该栏目需要登录且有访问权限才可以访问

如果您有访问权限,请直接 登录访问

如果您没有访问权限,请联系管理员申请开通

管理员联系邮箱:yun@hnwdkj.com