期刊论文

中文

基础医学与临床

1001-6325

2018

38

9

1231-1238

10.16352/j.issn.1001-6325.2018.09.004

81573965:国家自然科学基金 81403387:国家自然科学基金 2015DK3003:湖南省科技厅重点项目 2017CX30:创新基金

本校为第一机构

目的研究下丘脑-垂体-肾上腺(HPA)轴紊乱对焦虑性抑郁模型大鼠海马结构的影响,探讨焦虑性抑郁的潜在发病机制。方法将大鼠随机分为空白组、溶媒组、焦虑组、抑郁组和焦虑性抑郁组,每组12只。采用慢性束缚应激联合皮质酮注射方法建立焦虑性抑郁大鼠模型,连续21 d;造模后采用高架十字迷宫(EPM)、旷场实验(OFT)、强迫游泳实验(FST)评价大鼠焦虑和抑郁样行为;HE染色检测大鼠HPA轴各组织及海马病理变化;ELISA检测大鼠血浆中促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质酮(CORT)含量;Western blot检测大鼠海马糖皮质激素受体(GR)蛋白表达。结果焦虑性抑郁组大鼠进入开臂的时间、次数...

Objective To study the effect of hypothalamus pituitary adrenal(HPA) axis disorder on the hippocampal formation in rats with anxious depression and to explore its potential pathogenesis. Methods Rats were randomly divided into control group, vehicle group, anxiety group, depression group and anxious depression group with 12 rats in each group. Anxious depression rats were intervened by chronic restraint stress combined with corticosterone injection for continuous 21 d. Then, elevated plus maze (EPM), open field test (OFT), forced swimming test(FST)were used to evaluate the anxious and depressi...