期刊论文

Expression of caveolin-1 is enhanced in the differentiation of rat bone marrow mesenchymal stem cells into vascular smooth muscle cells

中文

基础医学与临床

1001-6325

2018

38

11

1517-1521

10.16352/j.issn.1001-6325.2018.11.002

81670268:国家自然科学基金 31371161:国家自然科学基金 16C1216:湖南省教育厅科学研究项目 B2016093:湖南省卫生计生委课题

本校为第一机构

目的 探讨大鼠骨髓间充质干细胞(BMSCs)向血管平滑肌细胞(VSMCs)分化过程中小窝蛋白-1(caveolin-1)的表达变化.方法 采用全骨髓培养法,体外分离培养SD大鼠BMSCs,成骨成脂成软骨检测干细胞的分化潜能,流式细胞仪鉴定干细胞表面标志物.体外TGF-β1诱导BMSCs向VSMCs分化,Western blot检测SM-MHC、SM22α、caveolin-1和myocardin的表达.结果 BMSCs在TGF-β1诱导下可分化为VSMCs,诱导培养组细胞caveolin-1、SM-MHC及myocardin表达的水平随诱导时间的增加而增强(P

Objective To explore the expression of caveolin-1 in the differentiation of rat bone marrow mesenchymal stem cells(BMSCs) into vascular smooth muscle cells(VSMCs). Methods BMSCs from SD rats were cultured by whole-bone marrow culture. Then, testing the differentiation potential of stem cells through adipogenic, osteoplastic and chondrogenic differentiation. BMSCs were induced to differentiate into VSMCs by transforming growth factor-β 1(TGF-β1) in vitro. Western blot was performed to evaluate the expression of SM-MHC, SM22α and caveolin-1. R...