期刊论文

中文

生物技术通报

1002-5464

2007

5

131-135

湖南省教育厅青年基金[湘财教指[2005]46号]（05B029）；

本校为其他机构

为了获得较多的原生质体用于原生质体融合,进行了酶解时间与浓度、稳定剂、菌龄、pH值等因素对革兰氏阳性菌株芽胞杆菌KR和革兰氏阴性菌株假单胞菌B13的原生质体制备影响的研究,结果表明：在以0.6mol/L蔗糖为稳定剂,pH8.0,溶菌酶浓度8mg/ml,37℃,酶解80min的条件下,芽胞杆菌KR原生质体得率为31.6%;在以.0.6mol/L NaCl为稳定剂,pH7.0,溶菌酶浓度6mg/ml,37℃,酶解45min假单胞菌B13原生质体得率最高,为95.2%。

In order to produce more protoplasts used in protoplast fusion,the studies on the effect of concentration of lysozyme,the time of lysozyme treatment,stabler,age of bacterium growing,pH on the formation of Gram-Positive Bacillus KR and Gram-Negative Pseudomonas B13 were conducted.The results showed that the highest formation rate of protoplast of KR was 31.6% under the condition of the concentration of sucrose 0.6mol/L,pH8.0,the concentration of lysozyme 8mg/ml,37℃,the time of lysozyme treatment 80min;the highest formation rate of protoplast of...