期刊论文

中文

中国中医基础医学杂志

1006-3250

2000

6

6

40-43

10.3969/j.issn.1006-3250.2000.06.016

国家自然科学基金!资助项目 （ No:3 9670 886）；

本校为第一机构

目的 :观察补肾醒脑解毒方对大鼠单疱病毒脑炎后脑内神经营养分子如 NGF、BDNF表达的调节作用。方法 :以 HSV- 1悬液经角膜划痕接种结膜囊内复制大鼠 HSV脑炎模型 ,用 Northern杂交与ELISA方法检测实验动物治疗前后脑内 NGF、BDNF的 m RNA与蛋白含量。结果 :HSV脑炎大鼠及正常大鼠服补肾醒脑解毒方后脑内 NGF、BDNF m RNA及蛋白均增高。结论 :补肾醒脑解毒方对 HSV脑炎后大鼠脑内 NGF、BDNF表达有促进作用 ,提示补肾醒脑解毒方对 HSV脑炎后遗症潜在着良好治疗作用。

Objective: Investigating the effects of Bushen Xingnao Jiedu Fang on expressions of NGF and BDNF in rat brain after infection of herpes simplex virus type 1 (HSV 1). Methods: Rats with 20 day age were anesthetized intravenously with pentobarbital sodium and their right conjunctival sac was infected with 50ul of virus suspension (containing about 10 4 TCID 50 HSV 1 viron) after the cornea scarified.The mRNAs of NGF and BDNF were detected with Northern hybridization, and the protein of NGF and BDNF were detected with ELISA. Results: (1) The expression of NGF and BDNF was decreased at the day 20 ...