期刊论文

Effect of Qiwei Baizhu Powder Combined with Yeast on the Intestinal Bacteria Diversity in Dysbacteriotic Diarrhea Mice

中文

中国中西医结合杂志

1003-5370

2018

38

1

66-70

10.7661/j.cjim.20171207.299

国家自然科学基金资助项目（No.81573951） 湖南省***卫生人才225工程培养项目 方剂学湖南省重点学科资助

本校为第一机构

目的探讨七味白术散与酵母菌联用对肠道细菌多样性的影响,为临床联用提供理论依据。方法48只SPF级KM小鼠,随机分为正常组、模型组、传统七味白术散组和25%超微七味白术散+ 25%酵母组,每组12只。采用抗生素建立小鼠菌群失调腹泻模型,给予抗生素造模菌群失调腹泻小鼠,分别灌胃25%超微七味白术散[0.04 g /(kg·d)超微七味白术散]+ 25%酵母菌[0.25 × 10~(10)个酵母/(kg·d)]、传统七味白术散[0.16 g /(kg·d)传统七味白术散]、等量无菌生理盐水,采集回肠肠道内容物,提取肠道微生物宏基因组DNA,引物经过聚合酶链式反应(polymerase chain reaction,PCR)扩增后进行核糖体DNA扩增片段限制性内切酶分析(...

Objective To observe Qiwei Baizhu Powder （QBP） combined with yeast on the diversity of the intestinal bacteria in dysbacteriotic diarrhea mice, thus providing theoretical evidence for clinical application. Methods Totally 48 specific pathogen free Kunming mice were randomly divided into the normal group, model group, traditional QBP group and 25% ultra-micro QBP ＋25% yeast group, twelve mice in each group. Mouse model of dysbacteriotic diarrhea was constructed with antibiotics by gastrogavage. Mice with the symptoms of diarrhea were then tre...