期刊论文

中文

中医药临床杂志

1672-7134

2022

34

03

463-468

湖南省研究生科研创新项目资助(省教育厅CX20200810) 湖南中医药大学研究生创新课题(校级、编码:2020CX65)。

本校为第一机构

目的:应用网络药理学及分子对接探讨肉苁蓉防治IUA的作用机制。方法:应用TCMSP和Uniprot数据库筛选出肉苁蓉的活性成分及靶基因,从GeneCards、OMIM、PharmGkb数据库中检索出疾病基因,得到肉苁蓉防治IUA的潜在靶点;运用Cytoscape软件构建“药物-成分-潜在靶点”网络图;通过STRING平台构建蛋白互作网络(PPI);对筛选的潜在靶点进行GO功能富集和KEGG通路富集分析,并进行分子对接验证,预测出肉苁蓉防治IUA的作用机制。结果:获得肉苁蓉活性成分5个,与IUA交集靶点117个,关键活性成分为槲皮素、β谷甾醇,主要作用于AKT1、CCND1、MAPK1、RELA、TNF、TP53等核心靶点。GO分析主要涉及纤维化、细菌病毒感染、...

Objective:To investigate the mechanism of Cistanche deserticola in the prevention and treatment of IUA by using network pharmacology and molecular docking.Methods:TCMSP and Uniprot databases were used to screen out the active components and target genes of Cistanche deserticola,and disease genes were retrieved from GeneCards,OMIM,and PharmGkb databases to obtain the potential targets of Cistanche deserticola for preventing and treating IUA;Cytoscape software was used to construct a“drug-component-potential target”.The protein interaction netw...