期刊论文

中文

神经损伤与功能重建

1001-117X

2020

15

5

271-273+284

10.16780/j.cnki.sjssgncj.20190297

No.81603512:国家自然科学基金 81874429:国家自然科学基金 No.20180364:湖南省卫生计生委科研课题 No.20200009:湖南省卫生计生委科研课题 No.19B440:湖南省教育厅科学研究项目 No.201818:湖南省中医药科研项目

本校为第一机构

目的:探讨NLRP3在匹罗卡品致癫痫大鼠模型中海马齿状回的表达及作用。方法:大鼠64只,随机选取12只为对照组,其余大鼠建立氯化锂-匹罗卡品癫痫模型,造模成功后随机分为1 d、7 d、14 d、28 d组,每组12只。ELISA检测大鼠血清IL-1β、TNF含量;免疫组化、RT-PCR检测大鼠海马组织齿状回NLRP3、Caspase-1在癫痫造模后不同时间点的表达水平。结果:7 d、14 d、28 d组血清中IL-1β、TNF的含量较对照组高（P

Objective:To explore the effect of NLRP3 on the hippocampal dentate gyrus in the pilocarpine-induced rat epilepsy model.Methods:Twelve rats were randomly selected from 64 adult SD rats as controls,and the others were induced by lithium-pilocarpine to create the epilepsy model.After model establishment,the rats were randomly divided into the 1 d,7 d,14 d,and 28 d groups(n=12 per group).The serum levels of IL-1βand TNF were detected with ELISA.The expression of NLRP3 and Caspase-1 in the hippocampal dentate gyrus was measured with immunohistoche...