期刊论文

Screening and Functional Analysis of Differentially Expressed CircRNA in Hippocampus of Rats with Epilepsy Induced by Lithium Chloride-Pilocarpine

中文

神经损伤与功能重建

1001-117X

2022

17

6

315-319

10.16780/j.cnki.sjssgncj.20201286

No.81874429:国家自然科学基金 No.20200009:湖南省卫生计生委科研课题 B2019047:湖南省卫生计生委科研课题 No.2019JJ80027:湖南省自然基金 2020JJ5294:湖南省自然基金 No.202145:湖南省中医药管理局重点项目 No.19B440:湖南省教育厅科学研究项目

本校为第一机构

目的:探究氯化锂-匹罗卡品诱导癫痫大鼠海马组织差异表达共价闭合、单链环状RNA(circRNA)及功能分析.方法:通过构建氯化锂-匹罗卡品癫痫大鼠模型,取癫痫大鼠及对照大鼠海马组织通过Illumina高通量测序平台进行转录组测序,并通过EdgeR包对circRNA进行差异分析、通过R语言进行GO及KEGG信号通路富集分析,通过Targetscan与miRanda软件进行circRNA与miRNA互作分析寻找circRNA核心调控靶标.结果:对照组及癫痫组大鼠海马的circRNA进行差异分析获得的262个差异表达circRNA,信号通路富集分析发现与胞蛋白修饰过程、蛋白质变性过程、树突形态调控、神经元分化调节、离子跨膜转运的调节等生物过程相关.并进...

Objective:To investigate the differential expression of circRNA and its function in hippocampal tissues of epileptic rats induced by lithium chloride-pilocarpine.Methods:The epileptic rat model was established using lithium chloride-pilocarpine.Hippocampal tissue of epileptic rats and control rats was collected for transcriptome sequencing by the Illumina high-throughput sequencing platform.The differences in circRNA were analyzed with the edgeR package.GO and KEGG pathway enrichment analysis was performed in R.Targetscan and miRanda were used for circRNA-miRNA interaction software analysis to...