期刊论文

Effect of Yiqi Huayu Jiedu Formula and Its Separates on Nude Mice Transplantation Tumor of HepG2 Cells and Expression of HIFla and E-cad

中文

辽宁中医杂志

1000-1719

2014

41

9

2004-2006

10.13192/j.issn.1000-1719.2014.09.090

国家自然科学基金资助项目(81001522) 湖南省自然科学基金课题(13JJ4138) 首届中国中西医结合学会-金港榄香烯中医药肿瘤研究基金资助(2010-F10)

本校为第一机构

目的:观察益气化瘀解毒方对人肝癌移植瘤及HIF1a、E-cad表达的影响。方法:建立人肝癌移植瘤模型,分为7组,分别以益气化瘀解毒方、黄芪、莪术、重楼、壁虎、顺铂药物干预21 d后,取瘤组织,行免疫组化检测。结果:（1）成瘤体积比较:给药后瘤体积及差值比较,各组与空白组均有显著性差异（P0.05）。结论:（1）益气化瘀解毒方全方较单味中药具有较强的抑瘤作用,其组方中药如莪术、重楼、壁虎均有一定程度抑瘤作用,莪术抑瘤作用较强;组方内主要单味中药发挥了协同抑瘤效应,使整方呈现出最强的抑瘤效应。（2）益气化瘀解毒方能抑制HIF1a表达,促进E-cad表达,可能通过阻断上皮间质转化而发挥抗肝癌转移侵袭...

Objective：To observe the effect of Yiqi Huayu Jiedu （YQHYJD） formula and its separate on nude mice transplanta- tion tumor of hepatoma cells and the expression of HIF1 a and E-cad. Methods ： Models of nude mice transplantation tumor are di- vided into 7 groups, treated by YQHYJD, astragalus, rhizoma zedoariae, paris polyphylla, gecko and cisplatin, after 21 days, immu- nohistochemical detection in tumor tissue. Results ： （ 1 ） The effect on tumor volume ： There was significant difference between the black and the other except Astragalus...