期刊论文

中文

辽宁中医杂志

1000-1719

2018

45

12

2473-2477

10.13192/j.issn.1000-1719.2018.12.003

国家自然青年科学基金项目(81202794) 湖南省教育厅课题(16B191).

本校为第一机构

目的:应用蛋白质组学技术探索脑泰方干预脑梗死大鼠血浆中的特异蛋白,筛选出药物作用的关键蛋白,为明确中药治疗脑梗死的作用靶点和作用机制研究提供依据。方法:建立MCAO大鼠模型,将脑梗死+脑泰方组、脑梗死组大鼠的血浆采用2-DE技术结合质谱分析进行蛋白质的比较分析,筛选出差异蛋白,运用生物信息学技术对差异蛋白质进行功能分析。结果:（1）对"脑梗死组"血浆与"脑梗死+脑泰方组"血浆的胶图扫描结果进行比较显示:有21个显著差异蛋白点;其中"脑梗死组"血浆中的5个蛋白质（肽）在"脑梗死+脑泰方组"血浆中不存在;另外有13个蛋白质（肽）在"脑梗死+脑泰方组"血浆中比在"脑梗死组"血浆中明显减少,有...

Objective:To explore the specific protein in Naotai Formula intervention in rats with cerebral infarction plasma by application of proteomics technology,search the key protein and provide the basis for the research target and mechanism of traditional Chinese medicine in the treatment of cerebral infarction.Methods:The rat model of MCAO was set up.Mass spectrometry analysis was used for plasma protein in cerebral infarction group and cerebral infarction + Naotai Formula group.Using 2-DE technology,the differential proteins were analyzed.Results:(1)Gel map scanning results of cerebral infarction...