期刊论文

Effectof Huagan Decoctionon Expressions of TGF-β1,CTGF and MAPK1 in HSC-T6 Cell

中文

辽宁中医杂志

1000-1719

2020

47

7

185-188

10.13192/j.issn.1000-1719.2020.07.052

湖南中医药大学校级科研基金(ZYYDX201738) 国家自然基金青年项目基金(81803641) 湖南省自然基金面上项目(2020JJ4069)。

本校为第一机构

目的 探讨化肝煎含药血清对大鼠肝星状细胞HSC-T6增殖与相关基因表达的影响.方法 采用正交试验法优选化肝煎舍药血清的制备,并以优化后的化肝煎含药血清干预HSC-T6,MTS法检测其对HSC-T6生长的抑制作用;qPCR法检测HSC-T6细胞中转化生长因子β1(transforming growth factor-beta 1,TGF-β1)、结缔组织生长因子(connective tissue growth factor,CTGF)、有丝分裂原活化蛋白激酶1(mitogen activated protein kinase,MAPK1) mRNA表达.结果 A1B2C2为优化方案,即给正常大鼠灌胃给药,末次给药后1h,采集血清;化肝煎含药血清体积分数为10％、20％和40％时可显著抑制HSC-T6的增殖(P＜0.01、0.001),并对TGF-...

Objective To investigate the effects of serum containing Huagan Decoction(SCHGD)on cell HSC-T6 proliferation and related genes expressions.Methods The orthogonal experiment was carried out to screen the preparative method of drug-containing serum.HSC-T6 was cultured in vitro and treated with SCHGD.MTS assay was used to evaluate the inhibitory effect of SCHGD on HSC-T6.The mRNA expressions of transforming growth factor-beta 1(TGF-β1),connective tissue growth factor(CTGF)and mitogen activated protein kinase(MAPK1)were determined by qPCR.Results ...