会议论文

Effects of Zuogui Jiangtang Yishen decoction on the expression of Rho, ROCK in podocyte of MKR mice with diabetic nephropathy

中文

2014

322-326

世界中医药学会联合会中医诊断学专业委员会成立大会暨第一届学术年会

世界中医药学会联合会中医诊断学专业委员会成立大会暨第一届学术年会论文集

2014-06-20

长沙

世界中医药学会联合会

本校为第一机构

　　目的 探讨左归降糖益肾方对2型糖尿病肾病(DN)小鼠足细胞Rho、ROCK表达的影响.方法 将40只MKR小鼠(骨骼肌特异性Insulin/IGF-1双受体缺失的转基因2型糖尿病)随机分为4组,空白组、DN组、中药组(左归降糖益肾方)、阻断剂组(Rho/ROCK信号通路特异性阻断剂Y-27632).其中DN组、中药组、阻断剂组MKR小鼠给予高脂饮食联合右侧肾切除.手术4周后各给药组开始给药,分别给药4周后,各组小鼠处死.电化学法检测血糖；自动生化仪检测血肌酐(Scr)及血尿素氮(BUN)；酶联免疫法(ELISA)检测尿白蛋白(UmAlb)含量；western blotting检测Rho、ROCK蛋白表达.结果 与空白组比较,DN组小鼠肾小球病变明显,小鼠血糖、Sc...

　　Objective To investigate the expression of Rho and ROCK, and the effects of ZuoguiJiangTangYiShen Decoction (ZGJTYS) intervention in MKR mice with Diabetic Nephropathy (DN).Methods Forty MKR mice (8 weeks old) who expressed dominant-negative IGF-1 receptor and insulin receptor in skeletal muscle were divided randomly into four groups as follows: negative control group, DN model group, ZGJTYS group and inhibitor group (Y-27632).Four weeks after operation, all mice were received drug intervention, and four weeks later, all mice were put to de...