期刊论文

Effect of Ziyin Huoxue Jiedu Decoction on VEC Injury Induced by AGEs Based on RAGE/NF-κB/mTOR Signaling Pathway

中文

湖南中医药大学学报

1674-070X

2017

37

7

719-722

10.3969/j.issn.1674-070X.2017.07.006

16K063:教育厅项目 201606:湖南省中医药科研项目 :中西医结合基础湖南省重点学科建设项目

本校为第一机构

目的 观察滋阴活血解毒方对糖基化终末产物(advanced glycosylation end products,AGEs)诱导人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)损伤的影响及RAGE/NF-κB/mTOR信号通路的变化,探讨该方对AGEs诱导静脉内皮细胞(vein endothelial cell,VEC)损伤的保护作用。方法 培养的HUVEC细胞以100 mg/ml的AGEs诱导损伤,随机分为7个组,除空白组外,其中3组分别加入RAGE抑制剂FPS-ZM1、NF-κB抑制剂PDTC和mTOR抑制剂雷帕霉素(RAP),余下3组加入各种抑制剂后加入50 mg/m L滋阴活血解毒方浓缩药液。以western-blot法检测RAGE和mTOR的表达,以免疫组化方法检测NF-κB核转移状况。结果 细胞...

Objective To observe the effect of Ziyin Jiedu decoction on the damage of human umbilical vein endothelial cell (HUVEC) induced by AGEs and the change of RAGE/NF-κB/mTOR pathway, and to explore the protective effect of this prescription on AGEs-induced VEC injury. Methods The damage of HUVEC cells were induced by 100 mg/mL AGEs and randomly divided into seven groups. Three of these groups excepted blank group were added RAGE inhibitors FPS-ZM1, NF-κB inhibitor PDTC and mTOR inhibitor rapamycin (RAP) respectively, and the remaining three group...