期刊论文

Establishment of Qi-asthenia and Phlegm-stasis Model in Type 2 Diabetic Rats with Myocardial Fibrosis

中文

中国中医急症

1004-745X

2016

25

6

953-956

10.3969/j.issn.1004-745X.2016.06.004

国家自然科学基金面上项目（81373551）； 教育部博士点博士导师课题（20134323110001）； 湖南省科技厅社会发展支撑计划重点项目（2014S2032）； 湖南省中医药科研计划项目（2014153）； 湖南省研究生创新课题（CX2015B332）； 中医诊断学国家重点学科建设项目（2013ZYZD04,2014-5,2014-28,ZYZD2015B32）； 湖南省学位与研究教育教学改革研究课题（JG2015B068）；

本校为第一机构

目的制备模拟人类发病过程的大鼠2型糖尿病心肌纤维化气虚痰瘀证模型。方法用雄性5周龄SD大鼠40只,随机分为正常对照组8只、高糖高脂组8只、模型组24只,高糖高脂喂养4周造成胰岛素抵抗后,正常对照组和高糖高脂对照组分组予以普食和高糖高脂饲料喂养。模型组于第5周予以腹腔注射2次1%小剂量STZ,2次之间间隔48 h,糖尿病造模成功后,随机继续高糖高脂喂养22周。通过心肌病理Masson染色、免疫组织化学染色测定CollagenⅠ、CollagenⅢ和β-actin,氯胺T法测定羟脯氨酸表达。结果模型组心肌病理切片及免疫组化结果显示存在心肌纤维化,正常组和高糖高脂组几乎没有;模型组TG、TC、LDL的值高于正常组;模型组...

Objective： To establish Qi-asthenia and phlegm-stasis model in type 2 diabetic rats with myocardial fibrosis to imitate Pathogenesis of human. Methods： 40 5-week-old SD rats were randomly divided into the control group（n = 8）,high-sugar and high-fat group（n = 8）,and the model group（n = 24）. High-sugar and high-fat group were fed for 4 weeks,causing insulin resistance. The control group and high-sugar and high-fat group were fed with normal food and high-sugar and high-fat food,respectively. In the fifth week,the model group received int...