期刊论文

中文

中国中西医结合杂志

1003-5370

2015

35

10

1218-1222

10.7661/CJIM.2015.10.1218

国家自然科学基金资助项目（No.81373641）； 湖南省科技厅课题资助项目（No.2011sk3104）； 长沙市科技局课题资助项目（No.K1301014-31）； 湖南省科技计划项目（No.2012SK3138）；

本校为第一机构

目的观察知柏地黄汤对解脲支原体(Ureaplasma urealyticum,UU)感染大鼠精液及生精细胞瞬时受体电位香草酸亚型(transient receptor potential family vanilloid subtype, TRPV)1、TRPV5表达的影响,探讨UU感染导致不育症的病理机制及知柏地黄汤的干预作用。方法4 ~5月龄SD大鼠60只,随机抽取45只,经膀胱接种UU悬液,建立UU睾丸感染动物模型。剩余15只同步注射生理盐水作为正常对照组,UU感染模型大鼠再随机分成模型对照组、阿奇霉素组、知柏地黄汤组,每组15只。知柏地黄汤组给予知柏地黄汤1 g/(kg ? d)灌胃,阿奇霉素组给阿奇霉素混悬液0.105 g/(kg ? d)灌胃,正常对照组、模型对照组给予等剂量生理盐...

OBJECTIVE: To observe the effect of Zhibai Dihuang Decoction (ZDD) on mRNA and protein expressions of transient receptor potential family vanilloid subtype 1 (TRPV1) and transient receptor potential family vanilloid subtype 5 (TRPV5) in Ureaplasma urealyticum (UU)-infected rat semens and spermatogenic cells, and to explore the pathomechanism of UU-infected infertility and the intervention of ZDD. METHODS: Totally 45 were randomly selected from 60 4-5 months old SD rats. UU testicular infected animal models were set up after bladder inoculation of UU suspension. The remaining 15 rats were simul...