Objective To investigate the biological theory and experimental foundation for transduction of exogenous genes into cultured Schistosoma japonicum (Sj) cells using an amphotropic retrovirus. Methods The homologous distribution, structure, and function of receptors for an amphotropic retrovirus, Rattus norvegicus (rRam-1) , were systematically analyzed and compared using bioinformatics methods. Afterwards, the cultured cells of 12-day-old Sj schistosomula infected with the amphotropic retrovirus containing exogenous gene E77. 43 were identified with PCR and RT-PCR in order to integrate and expr...
