期刊论文

Time-effect relationship and molecular mechanism of electroacupuncture for repair of gastric mucosal lesion

中文

中国针灸

0255-2930

2018

38

7

747-752

10.13703/j.0255-2930.2018.07.018

深圳市科技计划项目：JCYJ 20160406140612883 国家自然科学基金项目：81473751

本校为其他机构

目的:动态观察电针对胃溃疡模型大鼠胃黏膜损伤修复的影响,探讨电针治疗胃溃疡的时效关系和分子机制。方法:72只SD大鼠分为空白组、模型组、胃经穴组、对照点组,并按干预时间1、4、7 d分为3个亚组,每个亚组6只。采用乙醇灌胃方法制备胃溃疡大鼠模型,胃经穴组电针“足三里”“梁门”,对照点组电针“梁门”“足三里”外旁开5 mm处,每日1次,每次电针30 min。空白组和模型组用鼠板束缚但不进行电针处理,每日1次,每次30 min。用逆转录-聚合酶链反应(PR-PCR)法检测胃增殖细胞核抗原(PCNA)、P物质(SP)的表达,Western blot检测胃神经降压素(NT)的表达。结果:干预1 d后,模型组溃疡指数显著高于空白组(P

OBJECTIVE: To dynamically observe the effects of electroacupuncture (EA) on repair of gastric mucosal lesion in rats with gastric ulcer, and to explore the time-effect relationship and molecular mechanism of EA for gastric ulcer. METHODS: A total of 72 SD rats were randomly assigned to a normal group, a model group, a acupoint group and a sham acupoint group, and each group were further divided into a 1-day subgroup, a 4-day subgroup and a 7-day subgroup, 6 rats in each subgroup. The rat model of gastric ulcer was established by using intragastric administration of ethyl alcohol. The rats in t...