期刊论文

Guo-Min, Z.;Hui-Ping, L.

中文

数字中医药(英文)

2096-479X

2019

2

2

105-116

10.1016/j.dcmed.2019.09.005

We thank for the funding support from the National Natural Science Foundation of China (No. 81573956) Natural Science Foundation of Hunan Province (No. 2018JJ2297) Key Program of Chinese Medicine Science Research Plan of Hunan Province (No. 201612) Key Program of Scientific Research Fund of Hunan Provincial Education Department (No. 16A162) National College Students Innovation and Entrepreneurship Project (No. 201710541002) The Project of Research Learning and Innovative Experiment for College Students in Hunan (No. 2015217, No. 2015220, No. 2016284 and No. 2016281).

本校为第一且通讯机构

目的观察壮骨止痛方(ZGZTF)通过干预Wnt/β-catenin 信号通路拮抗剂SOST 治疗骨质疏松症的作用。方法从GEO 数据库中分析发现骨质疏松症患者和正常人的显著差异表达的基因SOST,并通过KEGG 分析出SOST 为Wnt 信号通路的拮抗剂。随后我们研究了壮骨止痛方对Wnt 信号通路中SOST 的影响,建立8 周龄的雌性SD 大鼠去势诱导骨质疏松症模型。壮骨止痛方灌胃处理12 周后,将大鼠分批处死,测定大鼠血清中碱性磷酸酶(ALP),骨钙素(BGP)和雌二醇(E2)的变化,观察右侧股骨的骨密度和组织形态学,测量腰椎的生物力学,以及SOST 的表达量。通过Western blot,RT-PCR 和免疫组化分析检测Wnt3a,β-catenin, LRP5, Runx2...

Objective: To observe the effects of Zhuang Gu Zhi Tong Formula (ZGZTF) on antagonist SOST in canonical Wnt/β-catenin signaling pathway in osteoporosis. Methods: We analyzed the differential genes of patients with osteoporosis and normal subjects from the GEO database and then found SOST with specific expression. We analyzed SOST as an antagonist of the Wnt signaling pathway by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Then we studied the effect of ZGZTF on SOST in Wnt signaling pathway. Osteoporosis model was induced by ovar...