Purification and characterization of bacteriocin F1, a novel bacteriocin produced by Lactobacillus paracasei subsp. tolerans FX-6 from Tibetan kefir, a traditional fermented milk from Tibet, China

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成果类型：

期刊论文

作者：

Miao, Jianyin;Guo, Haoxian;Ou, Yangwen;Liu, Guo;Fang, Xiang;...

通讯作者：

Cao, Yong

作者机构：

[Chen, Yunjiao; Fang, Xiang; Cao, Yong; Miao, Jianyin; Ke, Chang; Guo, Haoxian; Liao, Zhenlin; Zhao, Lichao; Liu, Guo] South China Agr Univ, Coll Food Sci, Guangzhou 510542, Guangdong, Peoples R China.

[Miao, Jianyin] Qinzhou Univ, Sch Ocean, Qinzhou 535000, Peoples R China.

[Ou, Yangwen] Hunan Univ Chinese Med, Sch Pharm, Changsha 410000, Hunan, Peoples R China.

[Cao, Yong] South China Agr Univ, Coll Food Sci, 483 Wushan Rd, Guangzhou 510542, Guangdong, Peoples R China.

通讯机构：

[Cao, Yong] S

South China Agr Univ, Coll Food Sci, 483 Wushan Rd, Guangzhou 510542, Guangdong, Peoples R China.

语种：

英文

关键词：

Bacteriocin;Lactobacillus paracasei subsp. tolerans;Purification characterization;Tibet kefir

期刊：

Food Control

ISSN：

0956-7135

年：

2014

卷：

页码：

48-53

DOI：

10.1016/j.foodcont.2014.01.041

基金类别：

Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31171768]

机构署名：

本校为其他机构

院系归属：

药学院

摘要：

A bacteriocin-producing lactic acid bacteria, FX-6, was isolated from Tibetan kefir using the agar well diffusion assay. Strain FX-6 was identified as Lactobacillus paracasei subsp. tolerans on the basis of 16S rDNA sequence analyses. This bacteriocin, which was designated bacteriocin Fl, was purified by a threestep purification procedure. The molecular mass of bacteriocin Fl was 2113.842 Da by MALDI-TOF MS analyses. It was also discovered to have blocked N-termini, hampering analysis by Edman degradation. Bacteriocin Fl exhibited a wide range of antimicrobial activity, strong heat stability (...

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Purification and characterization of bacteriocin F1, a novel bacteriocin produced by Lactobacillus paracasei subsp. tolerans FX-6 from Tibetan kefir, a traditional fermented milk from Tibet, China

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