期刊论文

Effect of Zuogui Jiangtang Shuxin Formula Medicated Plasma on Foaming and Apoptosis of Macrophage Induced by ox-LDL Through PERK/eIF2α/ATF4 Signaling Pathway

中文

南京中医药大学学报

1672-0482

2020

36

03

370-375

10.14148/j.issn.1672-0482.2020.370

81573956:国家自然科学基金 2018YFC1704300:国家重点研发计划 CX2018B471:湖南省研究生科研创新项目 2018ZYX44:湖南中医药大学中医学一级学科开放性基金项目

本校为第一机构

目的 探讨左归降糖舒心方含药血浆对氧化低密度脂蛋白(ox-LDL)诱导巨噬细胞泡沫化和凋亡的影响及其作用机制.方法 将巨噬细胞J774A.1随机分为正常组、模型组、牛磺熊去氧胆酸(TUDCA)组、左归降糖舒心方含药血浆组、西药联合含药血浆组,每组5个复孔.除正常组外其余各组以ox-LDL(100 mg/L)处理构建巨噬细胞泡沫化模型.正常组和模型组正常培养,其余各组分别用10％ 相应含药血浆培养24 h.CCK-8法比较各组J774A.1细胞增殖情况,计算细胞抑制率;油红O染色检测细胞内脂质蓄积水平;Western blot检测双链RNA样内质网激酶(PEPK)、活化转录因子4(ATF4)、真核翻译起始因子2α(eIF2α)、C/EBP环磷酸腺苷反应元...

OBJECTIVE To investigate the effect of Zuogui Jiangtang Shuxin Formula medicated plasma on ox-LDL-induced PERK/eIF2α/ATF4integrated stress response and activation of apoptosis key signaling molecules CHOP and Caspase-12.METHEOD J774A.1cells were randomly divided into 5groups:normal group,model group,TUDCA group,Zuogui Jiangtang Shuxin Formula medicated plasma group and western medicine combination(metformin+atorvastatin calcium)medicated plasma group.All the drug intervention groups were pretreated with ox-LDL to establish an experimental mode...