期刊论文

中文

湖南中医药大学学报

1674-070X

2017

37

8

823-826

10.3969/j.issn.1674-070X.2017.08.003

中西医结合基础湖南省“十二五”重点学科开放基金资助项目；

本校为第一机构

目的 探讨三黄地榆散对多重耐药铜绿假单胞菌的抑制效果,为其临床应用提供依据.方法 首先制备中药含药血清用于体外最小抑菌浓度(MIC)、最小杀菌浓度(MBC)测定.然后建立烫伤大鼠感染多重耐药铜绿假单胞菌模型,分别给予西药硫酸庆大霉素和中药三黄地榆散灌胃,正常组和模型组灌胃等容量的无菌生理盐水;在给药后的第3、7、10天检测血液中炎性细胞和炎性因子的水平.结果 体外抑菌实验显示,三黄地榆散含药血清与庆大霉素对多重耐药铜绿假单胞菌的MIC、MBC均为512μg/mL.体内实验结果显示,与模型组比较均能显著下调大鼠血清WBC、IL-1β、TNF-α的水平(P

Objective To investigate inhibitory effects of muitidrug resistant Pseudomonas aeruginosa by Sanhuang Diyu powder and provide the basis for its clinical application. Methods TCM serum was used for antibacterial experiment in vitro of minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The scald model rats infected by muitidrug resistant Pseudomonas aeruginosa were given with gentamicin sulphate and Sanhuang Diyu powder by gavage, re-spectively. Both the normal group and the model group were given with same volum...