期刊论文

中文

世界中西医结合杂志

1673-6613

2013

8

6

608-611

10.3969/j.issn.1673-6613.2013.06.023

湖南省教育厅科研项目（No.11C0961） 湖南中医药大学重点学科-病理学与病理生理学建设项目

本校为第一机构

目的 探讨山药多糖预处理对肾缺血再灌注损伤大鼠肾脏缺氧诱导因子-1α(HIF-1α)和血红素加氧酶-1(HO-1)表达的影响.方法 将健康雄性SD大鼠30只,随机分为假手术组、模型组、山药多糖预处理组,制备大鼠肾缺血再灌注损伤模型.术前1周,山药多糖预处理组每日予以山药多糖(200 mg·kg-1)灌胃,假手术组和模型组每日予以生理盐水(10 mL·kg-1)灌胃,再灌注6 h后检测各组大鼠血尿素氮(BUN)和血清肌酐(Scr)的含量,Western blot和RT-PCR法分别检测各组肾组织HIF-1α蛋白及mRNA、HO-1蛋白的表达水平.结果 山药多糖预处理组BUN、Scr含量明显低于模型组(P＜0.01),肾组织HIF-1α mRNA及蛋白、HO-1蛋白的表达明显...

Objective To explore the impacts of Chinese yam polysaccharide preconditioning （CYPP） on the expressions of HIF -1α and HO - 1 in the rats of renal ischemic reperfusion injury. Methods Thirty SD healthy male rats were randomized into three groups, a pseudo -operation group, a model group and a CYPP group. The rat model of renal ischemic reperfusion injury was prepared. In the CYPP group, the gastric perfusion of Chinese yam polysaccharide（200 mg · kg-1）was applied one week before operation. In the pseudo- operation group and the model gro...