An electrochemical strategy for tetracycline detection coupled triple helix aptamer probe with catalyzed hairpin assembly signal amplification

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成果类型：

期刊论文

作者：

Wang, Yonghong;Yao, Liu;Ning, Ge;Wu, Yaohui;Wu, Shun;...

通讯作者：

Liu, GQ

作者机构：

[Liu, GQ; Wu, Yaohui; Yao, Liu; Mao, Shaoming; Liu, Gao-Qiang; Wu, Shun; Wang, Yonghong] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Int Cooperat Base Sci & Technol Innovat Forest Re, Changsha 410004, Hunan, Peoples R China.

[Ning, Ge] Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Hunan, Peoples R China.

通讯机构：

[Liu, GQ ] C

Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Int Cooperat Base Sci & Technol Innovat Forest Re, Changsha 410004, Hunan, Peoples R China.

语种：

英文

关键词：

CHA signal amplification;Electrochemical;Host-guest recognition;TAP;Tetracycline

期刊：

Biosensors and Bioelectronics

ISSN：

0956-5663

年：

2019

卷：

143

页码：

111613

DOI：

10.1016/j.bios.2019.111613

基金类别：

CRediT authorship contribution statement Yonghong Wang: Methodology, Conceptualization, Writing - original draft, Formal analysis. Liu Yao: Methodology, Data curation, Validation. Ge Ning: Formal analysis, Writing - review & editing. Yaohui Wu: Software, Formal analysis. Shun Wu: Investigation, Formal analysis. Shaoming Mao: Project administration, Supervision, Writing - review & editing. Gao-Qiang Liu: Resources, acquisition.

机构署名：

本校为其他机构

院系归属：

国际教育学院

摘要：

Incorporating elements of triple-helix aptamer probes (TAP), catalyzed hairpin assembly (CHA) signal amplification and host-guest recognition, a novel 'signal-on' sensing strategy for sensitive electrochemical quantification of tetracycline (TC) was reported unprecedentedly. TAP was formed involving an aptamer loop, two-segment stems and a triplex oligonucleotide serving as trigger probe. Then, the trigger probe would be released from TAP once the target presented due to the conformational variation of TAP induced by aptamer binding event, spar...

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An electrochemical strategy for tetracycline detection coupled triple helix aptamer probe with catalyzed hairpin assembly signal amplification

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