会议论文

Study on the hydroscopicity of amino acids and variation of amino acids content in Yiganning extract by two purification methods

中文

2012

407-413

世界中医药学会联合会中药药剂专业委员会、中华中医药学会中药制剂分会学术年会暨“江中杯”中药制剂创新与发展论坛

世界中医药学会联合会中药药剂专业委员会、中华中医药学会中药制剂分会学术年会暨“江中杯”中药制剂创新与发展论坛论文集

2012-11-01

南昌

世界中医药学会联合会中药药剂专业委员会<&wdkj&>中华中医药学会中药制剂分会

本校为第一机构

目的：探讨乙肝宁复方氨基酸类吸湿性及壳聚糖絮凝澄清与醇沉工艺纯化乙肝宁后氨基酸含量变化规律.方法：采用阳离子交换树脂分离乙肝宁水提液中的氨基酸,测定吸湿百分率;将乙肝宁水提液经絮凝澄清与醇沉工艺处理后,用732＃阳离子交换树脂富集,采用异硫氰酸苯酯(PITC)柱前衍生,梯度洗脱,测定乙肝宁原浸膏及其两种纯化工艺处理后游离氨基酸含量.结果：乙肝宁复方总游离氨基酸吸湿性远远高于乙肝宁复方原浸膏;壳聚糖絮凝澄清法对乙肝宁复方水提液中精氨酸、赖氨酸的保留,明显优于醇沉法;而对于脯氨酸的保留,虽不如醇沉法,但仍有较高的保留率.结论：为揭示中药浸膏吸湿的本质原因及合理选择中药制剂...

　　Objective To explore the hydroscopicity of amino acids in Yiganning extract, and the discipline of amino acids content in Yiganning extract purified by chitosan flocculation clarification and alcohol precipitation.Methods Amino acids were obtained from Yiganning aqueous extract by the methods of separation and purification, such as cation exchange resin, and measure its percentage moisture.Amino acids of Yiganning extract purified by two purification methods and cation exchange resin were determined by pre-column derivatization RP-HPLC usin...