期刊论文

Dept. of Microbiol. and Immunol., Hunan College of TCM, China

中文

中华微生物学和免疫学杂志

0254-5101

2004

24

7

549-551

10.3760/j:issn:0254-5101.2004.07.013

国家自然科学基金

本校为第一机构

目的研究治喘贴对卵清蛋白所致实验豚鼠哮喘体内嗜酸粒细胞(EOS)凋亡及对白细胞介素(IL-5)的调节作用.方法将实验动物随机分为正常对照组、哮喘模型组、代温灸膏治疗组、治喘贴治疗组.经外贴治疗18 d,收集支气管肺泡灌洗液(BALF)并采集心脏血,采用流式细胞仪、免疫组织化学(TUNEL)和酶联免疫吸附(ELISA)法分析血中EOS凋亡和BALF中EOS凋亡数及IL-5含量.结果哮喘豚鼠血和BALF中EOS凋亡数、IL-5含量均高于正常对照组动物(P均＜0.01),治喘贴治疗豚鼠IL-5和EOS均明显低于哮喘模型组(P均＜0.01).结论治喘贴能促进哮喘豚鼠体内EOS凋亡,并降低IL-5的水平,从而改善哮喘模型豚鼠的气道变态性炎症反应.

Objective: To investigate the effect of Zhichuantie on eosinophils(EOS) and interleukin-5(IL-5) of asthma in experimental animals (guinea pigs) after being infected with ovalbumin. Methods: Experiment animals were randomly divided into four groups, control group, asthma group, Daiwenjiugao treatment group and Zhichuantie treatment group. After 18 days of treatment, the bronchus alveoli lavage fluid (BALF) and heart blood of guinea pigs were collected. Then we assayed the apoptosis of EOS in blood, the quantity of EOS and the level of IL-5 in BALF through the flow cytometry(FCM), terminal deoxy...