Objective To establish the animal model of chronic fatigue syndrome which comply with Chinese medicine characteristics. Methods The animal model of chronic fatigue syndrome was established by using the Chinese medicine complex stimulative factors such as physical fatigue, spiritual fatigue and sexual fatigue. The BALB/c mice were given the swimming combined with clipping tails and putting male and female into one cage different stimulation, and were compared with the swimming group in the observation of general condition and weight, and the det...