期刊论文

中文

中国药房

1001-0408

2017

28

22

3044-3047

10.6039/j.issn.1001-0408.2017.22.06

国家自然科学基金资助项目（No.81303004） 湖南省科技厅资助项目（No.2009RS3018） 湖南省教育厅资助项目（No.09C727）

本校为第一机构

目的:研究左归丸对环磷酰胺(CTX)致卵巢早衰(POF)小鼠卵巢组织中缝隙连接蛋白43(Cx43)表达的影响,探讨左归丸治疗化疗性POF的作用机制.方法:将72只小鼠按体质量随机分为空白组、模型组、戊酸雌二醇片组(阳性对照,0.00013 g/kg)和左归丸低、中、高剂量组(13.65、40.95、122.85 g/kg),每组10只.除空白组外,其余各组小鼠均ip CTX复制POF模型,每天1次,连续20 d;并同时ig相应药物,每天1次,连续30 d.末次给药2 h后,分别采用反转录-聚合酶链反应法和Western blot法检测卵巢组织中Cx43 mRNA和蛋白表达;并采用免疫组化法检测卵巢组织中Cx43蛋白的分布.结果:与空白组比较,模型组大鼠卵巢组织中Cx43 mRNA及...

OBJECTIVE:To study the effect of Zuogui pill on the expression of gap junction protein 43(Cx43)in ovarian tis-sue of mice with premature ovarian failure(POF)induced by intraperitoneal injection of cyclophosphamide(CTX),and explore its mechanism in the treatment of chemotherapy-induced POF. METHODS:72 mice were randomly divided into blank group,model group,Estradiol valerate tablet group (positive control,0.00013 g/kg),Zuogui pill low-dose,medium-dose,high-dose groups (13.65,40.95,122.85 g/kg)by body mass,10 in each group. Except for blank group,other groups were reduce POF model by ip CTX,once...