期刊论文

中文

中国临床药理学与治疗学

1009-2501

2016

21

2

125-129

湖南中医药大学研究生科研创新项目校行研字〔2014〕15号 湖南中医药大学大学生创新项目（2012-03） 湖南中医药大学重点学科资助（湘教通[2011]号）

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目的:建立枳椇子解酒方护肝活性成分最佳提取工艺,考察最佳提取工艺下该方低、中、高剂量的护肝药效。方法: 以乙醇浓度、料液比、粉碎度、提取时间为考察因素,以二氢杨梅素、齐墩果酸、浸膏得率为指标,采用正交试验设计,确定枳椇子解酒方护肝活性成分最佳提取工艺。建立急性酒精性肝损伤小鼠模型,考察最佳提取工艺下该方低、中、高剂量对血清ALT、AST、总胆固醇(CHO)活性及肝脏系数的影响。结果: 枳椇子解酒方最佳提取工艺为10目药材,50%乙醇,浸泡1 h,提取次数为2次,料液比为1∶10、1∶8,提取时间2.5、2.0 h。与模型组比较,阳性对照组、解酒方低、中剂量组肝脏系数、血清AST含量均显著降低(P

AIM: To study the optimum extraction process in Hovenia dulcis Thunb jiejiu recipe hepatoprotective constituents. METHODS: The ethanol concentration, solid-liquid ratio, grind degree and extraction time were taken as investigative factors, with dihydromyricetin, oleanolic acid, extractive yield as the index, by orthogonal experimental design,to determine the optimum extraction process. The acute alcoholic liver injury model was established, the influences of low, medium and high doses of Hovenia dulcis Thunb jiejiu recipe on serum ALT, AST, CHO...