期刊论文

中文

中国临床药理学与治疗学

1009-2501

2018

23

4

361-369

10.12092/j.issn.1009-2501.2018.04.001

国家自然科学基金面上项目（81473581） “中西医结合防治心脑血管疾病的相关基础研究”湖南省科技创新团队（2013年） “中医药防治心脑血管疾病基础研究”湖南省自然科学创新群体基金（2013年）

本校为第一机构

目的:探讨黄芪和当归不同配伍对大鼠血管内皮损伤后血管内膜增生中血管平滑肌细胞(VSMC)表型转化、细胞周期和磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号途径的影响。方法:雄性大鼠制备腹主动脉球囊导管损伤血管内皮模型,设计黄芪和当归单用及不同比例配伍,对内皮损伤模型大鼠灌胃给药,干预14 d后取腹主动脉,检测腹主动脉增生内膜中VSMC表型转化、细胞周期相关蛋白表达和PI3K/Akt信号通路相关蛋白的表达。结果:球囊导管损伤主动脉内皮后,可引起VSMC表型转化及VSMC增殖从而导致血管内膜增生。当归、黄芪、黄芪-当归1∶ 1配伍、黄芪-当归5∶ 1配伍可增强血管增生内膜中平滑肌α肌动蛋白(SM α-ac...

AIM: To investigate the effects of different Astragalus-Angelica combination on vascular smooth muscle cells (VSMC) phenotype transformation, cell cycle and PI3K/AKT signal pathway of vascular intimal hyperplasia in rats. METHODS: A model of intimal hyperplasia of thoracoabdominal aorta was established by balloon catheter injury in male Sprague-Dawley rat,and the model rats were administrated Astragalus and Angelica with single use or different ratios for 14 days by means of intragastric administration. Then the thoracoabdominal aorta was taken...