期刊论文

Effects of curcumin on proliferation of VSMCs and expression of PTEN induced by PDGF-BB

中文

中国临床药理学与治疗学

1009-2501

2019

24

12

1358-1363

10.12092/j.issn.1009-2501.2019.12.007

侗医药研究湖南省重点实验室项目(2015TP1020-04) 侗医药研究湖南省重点实验室平台建设项目(2017CT5025) 湖南省科技厅项目(2018SK4006) 湖南省自然科学基金青年项目(2019JJ50426)。

本校为其他机构

目的:研究在血小板源性生长因子( platelet- derived growth factor-BB,PDGF-BB)诱导下姜黄素对血管平滑肌细胞( vascular smooth muscle cell,VSMCs)增殖以及人第10号染色体缺失的磷酸酶及张力蛋白同源基因( phosphatase and tensin homology deletedon chromosome ten,PTEN)的影响,为侗族药物“姜黄”的临床应用提供更丰富的实验依据。方法:用20 ng /mL PDGF-BB孵育大鼠VSMCs 24 h,建立细胞增殖模型。MTT和CCK-8法观察姜黄素对VSMCs的细胞活性的影响,流式细胞术检测细胞周期,细胞划痕实验观察细胞迁移力,RT-PCR和Western blot观察PTEN mRNA、PTEN蛋白以及磷酸化PTEN( p-PTEN)蛋白的表达情况。...

AIM: To study the effects of curcumin on the proliferation of vascular smooth muscle cells ( VSMCs) and the deletion of phosphatase and tensin homology deletedon chromosome ten ( PTEN) induced by platelet-derived growth factor-BB ( PDGF-BB) and to provide more abundant experimental basis for the clinical application of Dong nationality medicine " Curcuma longa". METHODS: The proliferation model of VSMCs was established by incubating with PDGF-BB ( 20 ng /mL) for 24 h. The cell activity was observed by MTT and CCK-8,cell cycle and cell migration...