期刊论文

Roles of Prunella vulgaris L.in the proliferation and migration of vascular smooth muscle cells and its molecular mechanisms

中文

中国临床药理学与治疗学

1009-2501

2019

24

9

977-982

10.12092/j.issn.1009-2501.2019.09.003

81673722:国家自然科学基金 81603600:国家自然科学基金 16A156:湖南省教育厅重点项目 20160212:湖南省重点实验室第二批开放基金 18B252:湖南省教育厅优秀青年项目 2018JCYX04:湖南中医药大学基础医学一流学科开放基金 2018JCYX05:湖南中医药大学基础医学一流学科开放基金

本校为第一机构

目的:探讨夏枯草水提取物对血管紧张素II诱导的血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖作用及对Daxx/PTEN/NF-κB信号通路的影响。方法:培养VSMCs,体外用血管紧张素II (angiotensin II,Ang-II) (1 μmol/L)诱导细胞增殖模型,MTT和BrdU法分别检测夏枯草水提取物不同浓度(10、20、40、80、160 μg/mL)作用24 h后对VSMCs活性和增殖的影响、划痕法观察细胞迁移力,Western blot检测细胞中Daxx、PTEN、NF-κB蛋白表达。结果:与对照组比较,模型组VSMCs活力及细胞迁移能力明显增强;同时细胞中Daxx、PTEN蛋白表达明显降低,NF-κB蛋白显著上调;与模型组比较,20、40、80 μg/mL夏枯草水提...

AIM:To investigate the effects of Prunella vulgaris L.(PV) on Ang II-induced proliferation of VSMCs and the effect of Daxx/PTEN/NF- κB signaling pathway.METHODS:The VSMCs line was cultured,and the cell proliferation model was induced by Ang-II (1 μmol/L) in vitro.The MTT and BrdU methods were used to detect the effects of VSMCs activity and proliferation by different concentrations of PV (10,20,40,80,160 μg/mL) for 24 h.Cell migration was observed by scratch method,and Daxx,PTEN and NF-κB protein expression was detected by Western blot.RESU...