期刊论文

中文

中国临床药理学与治疗学

1009-2501

2022

27

7

739-746

10.12092/j.issn.1009-2501.2022.07.003

2020JJ5435:湖南省自然科学基金 2019XJJJ033:湖南中医药大学科研基金重点项目

本校为第一机构

目的:探究氟非尼酮(fluorofenidone, AKF-PD)对二乙基亚硝胺所致大鼠肝损伤的保护作用及对肝细胞中TGF-β1/Smads通路的影响。方法: 55只雄性Sprague Dawley(SD)大鼠随机分为3组:模型组(DEN组,n =20),灌胃给予10 mg/kg DEN,每周5次至14周停药;对照组(Control组,n =20),灌胃给与同模型组等体积的生理盐水;治疗组(DEN+AKF-PD组,n =15),在造模4周后,灌胃给与500 mg/kg AKF-PD,每天一次,至14周停药。实验结束后,麻醉,脊椎脱臼法处死大鼠。肝脏组织进行Masson染色以观察胶原沉积;提取并鉴定原代肝细胞,测定肝细胞中α-平滑肌肌动蛋白(α-SMA)、转化生长因子-β1(TGF-β1)、Smad3、Smad7 mRNA的水平,以...

AIM:To explore the protective effect of fluorofenidone(AKF-PD) on diethylnitrosamine-induced liver injury in rats and its inhibition of the TGF-β1/Smads pathway in hepatocytes.METHODS:Fifty-five male Sprague Dawley(SD) rats were randomly divided into three groups:model group(DEN group,n=20) were gavaged with DEN(10 mg/kg),5 times for 14 weeks;control group(n=20) were gavaged with saline with the same volume of the model group;treatment group(DEN+AKF-PD Group,n=15),after 4 weeks of modeling,they were gavaged with AKF-PD(500 mg/kg) daily,and sto...