期刊论文

中文

中华老年心脑血管病杂志

1009-0126

2010

12

7

645-647

10.3969/j.issn.1009-0126.2010.07.021

湖南省研究生科研创新基金项目（CX2009B170）；

本校为第一机构

目的 探讨健脾补土法对大鼠脑缺血再灌注后神经细胞周围层黏连蛋白(LN)的降解机制及其脑保护作用.方法 38只大鼠随机分为:假手术组8只,模型组10只,健脾补土传统汤剂组10只,健脾补土超微剂型组10只,大脑中动脉栓塞再通法建立脑缺血再灌注模型.进行神经功能症状评分,脑组织病理形态学观察,采用TUNEL法检测凋亡神经元,免疫组织化学法检测LN和基质金属蛋白酶9(MMP-9)阳性表达.结果 与假手术组比较,模型组神经功能症状评分、细胞凋亡指数、MMP-9阳性细胞表达数明显升高,LN表达明显降低(P＜0.01).与模型组比较,健脾补土传统汤剂组和健脾补土超微剂型组神经功能症状评分、细胞凋亡指数、MMP-9阳性细胞表...

Objective To investigate the effects of Tonifying Spleen therapy on perineuronal laminin（LN） degradation and its protective effect on cerebral function in cerebral ischemia-reperfusion （I/R） rats. Methods Thirty-eight rats were randomly divided into 4 groups,i, e. sham operation group,model group, traditional Tonifying Spleen group and ultramicro-dose Tonifying Spleen group. The model of cerebral I/R was formed by middle cerebral artery occlusion and recanalization. Neural function score was evaluated at 48 hours after cerebral reperfusion....