期刊论文

Content determination of ferulic acid in Xintong granules by HPLC

中文

医药前沿

2095-1752

2019

9

20

48-49

湖南省中医药科研基金项目(项目编号:201616).

本校为第一机构

目的:建立心痛颗粒中主药川芎药材中阿魏酸的含量测定方法.方法:采用高效液相色谱法测定阿魏酸含量,以Agilent Eclipse XD3-C18(250mm×4.6mm,5μm)为色谱柱;流动相为甲醇-1％ 醋酸溶液(30:70);流速1ml/min;检测波长321nm;柱温25℃.结果:阿魏酸进样量在0.048～0.24μg范围内与峰面积呈良好的线性关系(r=0.9999).平均回收率为99.26％(RSD=1.25％,n=9).结论:所建立的阿魏酸含量测定方法稳定、灵敏、准确、易操作,可作为心痛颗粒的质量控制.

Objective To establish a method for determination of ferulic acid in Xintong granules.Methods Determination of ferulic acid by HPLC.The determination was performed on Agilent Eclipse XD3-C18 column with mobile phase consisted of methanol-1% acetic acid solution(30:70) at the flow rate of lml/min. The detection wavelengths was at 321nm.The column temperature was at 25℃.Results The calibration curve of ferulic acid was in good linearity over the range of 0.048~0.24μg(r=0.9999).The average recovery was 99.26%,with RSD of 1.25%(n=9).Conclusion Th...